The Accuri C6 Flow Cytometer allows for rapid analysis of cell samples and provides information on
cell morphology, composition of a mixed sample, and expression of surface
proteins. The small footprint and incredible ease of use make the Accuri an
excellent instrument to train students on the theory and application of Flow Cytometry,
in addition to being a heavily used, and productive research instrument.
Hitachi TM3000 is a tabletop Scanning Electron Microscope which allows for
detailed analysis of the surface topography of virtually any material at an
extremely high magnification. While traditional SEM’s require extensive sample
preparation and intensive training, the Hitachi TM3000 is an incredibly
flexible platform that can be quickly mastered by students and used to analyze
un-prepared samples in real-time, thus making this high level technology that
has broad applications for Biology, Chemistry, and Physics, accessible and
The Zeiss AxioObserver D1
Widefield Fluorescence Microscope has
been a workhorse for nearly 20 years at USF and has produced images used in a
number of publications, however with the opening of the FJ Microscopy center it
was fully rebuilt and upgraded, it is now fully automated and run by the new
Zeiss software Zen. The excellent optics on this instrument allow for analysis and imaging of live and fixed cell
samples using bright-field, DIC, and fluorescence.
The Zeiss Laser Scanning
Confocal Microscope is the star of the Fletcher Jones Microscopy center, the
confocal scan head allows for the acquisition of
high-resolution optical images with depth selectivity, this greatly enhances
our understanding of processes on the subcellular level and is an excellent
research tool. The LSM700 is fully automated, and run using Zen software.
Zen (blue and black):
Both Zeiss microscopes have Zen software, in addition to an offline workstation
with a full Zen license. Many advanced modules are available for specialized
acquisition and data analysis.
Image J: The gold
standard for image analysis is available on the offline workstation.
BD Accuri: This
software runs acquisition and analysis on the Accuri C6 FlowCytometer.
FloJo: Flow cytometry
data analysis software.
Microsoft Office Suite 2013: Available on all computers.